Question

microscope study guide

1. what is the magnification of the eyepiece?
2. what is the total magnification of the following objective lenses with the eyepiece?

a. medium:
b. low:
c. high:

1. which adjustment knob should you use on the high power?
2. how do you prevent the slide from falling off the stage?
3. which adjustment knob is used for bringing the specimen into focus?
4. which adjustment knob is used for sharpening the image of the specimen after it is focused?
5. what is the function of the diaphragm?
6. how do you change the objective lenses?
7. what power must be in place when you first find an image in the microscope?
8. ** why do we call the microscope a compound light microscope?
9. how is a wet - mount slide prepared?
10. if an organism moves on the glass slide to the right and up, in what direction did it move in the field of view?

Explanation:

1. The magnification of the eyepiece is typically 10x.
2. Total magnification = Eyepiece magnification × Objective - lens magnification. Medium - power objective (10x) gives 100x total magnification, low - power objective (4x) gives 40x total magnification, high - power objective (40x) gives 400x total magnification.
3. On high power, use the fine - adjustment knob.
4. Use stage clips to prevent the slide from falling off the stage.
5. The coarse - adjustment knob is used to bring the specimen into initial focus.
6. The fine - adjustment knob is used to sharpen the image after it is focused.
7. The diaphragm controls the amount of light reaching the specimen.
8. Rotate the nosepiece to change the objective lenses.
9. Start with the low - power objective to first find an image.
10. It is called a compound light microscope because it has two sets of lenses (eyepiece and objective lenses) and uses light to view specimens.
11. To prepare a wet - mount slide: place a drop of water on the slide, place the specimen in the water, and cover with a coverslip.
12. Images seen through a microscope are inverted. So if an organism moves right and up on the slide, it appears to move left and down in the field of view.

Answer:

1. Typically 10x
2. a. 100x; b. 40x; c. 400x
3. Fine - adjustment knob
4. Use stage clips
5. Coarse - adjustment knob
6. Fine - adjustment knob
7. Controls the amount of light reaching the specimen
8. Rotate the nosepiece
9. Low - power objective
10. Has two sets of lenses and uses light to view specimens
11. Place a drop of water on the slide, place the specimen in the water, cover with a coverslip
12. Left and down