Question

multiple choice question
which statement best describes specimen preparation for tem?

the specimen is suspended in a semi - viscous medium within a depression - slide for viewing (often a stain is added).
the specimen is pre - stained with fluorochromatic molecules then fixed onto a slide.
the specimen is embedded in a matrix and cut into extremely thin slices.

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Explanation:

Transmission Electron Microscopy (TEM) requires electrons to pass through the specimen, so samples must be ultrathin. Embedding in a matrix (like resin) and sectioning into nanometer-thick slices is the standard preparation method. The other options describe preparation for light microscopy (first option) or fluorescence microscopy (second option).

Answer:

The specimen is embedded in a matrix and cut into extremely thin slices.