Question

question 13
8 parts — of 4 points
in pasteurs experiment to test the hypothesis of spontaneous generation, why did he boil the broth in both flasks?
to attract dust particles to each flask
to provide nutrients to stimulate the growth of microorganisms
to kill any existing organisms in the broth
to expose the broth to a source of organisms

Explanation:

Pasteur's experiment on spontaneous generation aimed to show that life doesn't arise spontaneously. Boiling the broth is a sterilization method. Option 1 is wrong as boiling doesn't attract dust. Option 2 is wrong because boiling doesn't provide nutrients for growth (and in fact kills microbes). Option 4 is wrong as boiling doesn't expose to organisms; the flask design (swan - neck) was for that. Boiling kills existing organisms in the broth to ensure that any later growth comes from outside, not spontaneous generation.

Answer:

C. To kill any existing organisms in the broth