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Question
a scientist wants to be able to artificially produce a protein that is produced in the livers of frogs. the scientist has located all of the mrna produced in the liver and produced the cdna. what should the scientist do to find the gene that produces this protein? a. mix the cdna with the liver dna. b. produced rna from the cdna. c. use reverse transcriptase on the cdna. d. insert the cdna into a bacteria sample.
To produce the protein, the scientist needs to express the gene. Inserting the cDNA (complementary DNA made from mRNA) into a bacteria sample allows for gene - expression in the bacteria. Bacteria can then use the cDNA to produce the desired protein. Mixing with liver DNA won't help in isolation or expression. Producing RNA from cDNA is not the next step for protein production. Using reverse - transcriptase on cDNA is not necessary as cDNA is already made from mRNA.
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D. Insert the cDNA into a bacteria sample