Question

we need to prepare a master mix with the reagents for each reaction. what is included in the master mix?
a) adh, nad⁺, and nadh
b) ethanol, nadh, and buffer
c) ethanol, nad⁺, and buffer
d) adh, nad⁺, and ethanol

Explanation:

This refers to the alcohol dehydrogenase (ADH) reaction, where ADH catalyzes the oxidation of ethanol using NAD⁺ as a cofactor, with a buffer to maintain optimal pH. A master mix contains shared reagents for replicate reactions; NADH is a product, not a starting reagent, so it is excluded.

Answer:

d) ADH, NAD⁺, and Ethanol