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Question
which enzyme in the graph has the higher km and affinity? b has a high km, but a has a low affinity. a has a high km, but b has a low affinity. a has a high km, but b has a high affinity. b has a high km, but a has a high affinity.
Step1: Recall Km - affinity relationship
Km is the substrate concentration at which the reaction rate is half of Vmax. A lower Km indicates a higher affinity of the enzyme for the substrate.
Step2: Analyze the graph
From the graph, Enzyme B reaches half - of its Vmax at a lower substrate concentration compared to Enzyme A. So, Enzyme B has a lower Km and thus a higher affinity. Enzyme A has a higher Km and lower affinity.
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A has a high Km, but B has a high Affinity.