QUESTION IMAGE
Question
- what is the recipe to make 5000 ul of a 1:100 dilution?
- what is the recipe to make 8 ml of a 1:8 dilution?
- if my 1:100 diluted sample has a concentration of 0.4%, what was the concentration of the original sample?
- if my 1:10 diluted sample has a concentration of 56 g/ml, what was the concentration of the original sample?
- if i dilute my sample 1:100 then 1:10, what is the total dilution relative to the original?
- if i dilute my sample 1:5 then 1:4, what is the total dilution relative to the original?
- if i want to make 100 ul of a 2% red dye 40 solution, how much red dye 40 should i include?
- if i want to make 600 ul of 17% red dye 40 solution, how much water should i add?
- how do i write 1,500 in scientific notation?
- how do i write 0.00067 in scientific notation?
- what is 2.37 x 10^2 written fully out?
- what is 7.6 x 10^-3 written fully out?
- if my 1:10 diluted sample has a concentration of 5.5%, what was the concentration of the original sample?
- if my 1:5 diluted sample has a concentration of 17%, what was the concentration of the original sample?
Question 14
Step1: Recall dilution formula
For a dilution, the ratio of the volume of the stock solution ($V_1$) to the volume of the final solution ($V_2$) is equal to the inverse of the dilution factor. The dilution factor ($DF$) for a 1:100 dilution is 100.
Step2: Calculate volume of stock solution
We know $V_2 = 5000\ \mu L$ and $DF = 100$. Using the formula $V_1=\frac{V_2}{DF}$, we have $V_1=\frac{5000}{100}=50\ \mu L$. The volume of the diluent (usually water) is $V_2 - V_1=5000 - 50 = 4950\ \mu L$.
Step1: Identify dilution factor
For a 1:8 dilution, the dilution factor ($DF$) is 8.
Step2: Calculate volume of stock solution
We know $V_2 = 8\ mL=8000\ \mu L$. Using the formula $V_1=\frac{V_2}{DF}$, we get $V_1=\frac{8000}{8}=1000\ \mu L = 1\ mL$. The volume of the diluent is $V_2 - V_1=8000 - 1000 = 7000\ \mu L=7\ mL$.
Step1: Use dilution - concentration relationship
The concentration of the original sample ($C_1$) and the diluted sample ($C_2$) are related to the dilution factor ($DF$) by the formula $C_1 = C_2\times DF$. For a 1:100 dilution, $DF = 100$ and $C_2 = 0.4\%$.
Step2: Calculate original concentration
$C_1=0.4\%\times100 = 40\%$
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Take 50 $\mu L$ of the stock solution and add 4950 $\mu L$ of diluent.