Question

1. using your beers law plot, determine the molar concentration of glucose in the unknown sample.

hoch₂(choh)₄cho + h₂n - c₆h₄ - ch₃ ⇌ hoch₂(choh)₄ch = n - c₆h₄ - ch₃ + h₂o
glucose o - toluidine blue - green complex

Explanation:

Step1: Recall Beer's law

Beer's law is $A = \epsilon l c$, where $A$ is absorbance, $\epsilon$ is molar - absorptivity, $l$ is path - length, and $c$ is molar concentration.

Step2: Use the Beer's law plot

On the Beer's law plot, the absorbance ($A$) of the unknown sample is measured. The plot has a linear relationship $A = mc + b$ (where $m=\epsilon l$ and $b = 0$ for a well - calibrated plot passing through the origin). We find the absorbance value of the unknown on the y - axis of the plot and then read the corresponding molar concentration ($c$) value on the x - axis.
However, since no data about the absorbance value of the unknown sample from the plot or the equation of the Beer's law plot is given in the problem, we cannot calculate a numerical value. But the general procedure is as described above.

Answer:

Cannot be determined without absorbance data from the Beer's law plot.