Question

1. explain the importance of washing out the micro wells after each step of elisa.

Explanation:

Washing the micro - wells after each step in ELISA removes unbound substances like excess antigens, antibodies, and enzyme - conjugates. This ensures that only specifically bound components remain for the next step, reducing background noise and false - positive results, thus increasing the accuracy and specificity of the assay.

Answer:

Washing out the micro - wells after each step in ELISA is important to remove unbound substances, reduce background noise, and increase the accuracy and specificity of the assay.