Question

given the standard curve below, what is the concentration of a sample with * 1 point an absorbance of 0.8? absorbance, 595 nm protein concentration, mg/l 2500 mg/l 213 mg/l 1175 mg/l 500 mg/l

Explanation:

Step1: Identify the relationship

The graph shows a linear relationship between absorbance and protein - concentration.

Step2: Locate the absorbance value

Locate the absorbance value of 0.8 on the y - axis (Absorbance, 595 nm).

Step3: Find the corresponding concentration

Draw a horizontal line from the absorbance value of 0.8 until it intersects the standard - curve. Then draw a vertical line from the intersection point to the x - axis (Protein concentration, mg/l). Reading from the x - axis, the corresponding protein concentration is approximately 1175 mg/l.

Answer:

1175 mg/l