Question

multiple choice question
in a confocal microscope, what removes stray light from parts of the specimen that lie above and below the plane of focus?

aperture
ocular
coarse adjustment
objective

Explanation:

In confocal microscopy, a pinhole aperture acts as a spatial filter. It blocks out-of-focus light (stray light from areas above/below the focal plane) while only allowing light from the focused plane to pass through, producing a sharp, high-contrast image. The ocular is for viewing, coarse adjustment changes focus position, and the objective magnifies the specimen, none of which perform this light-blocking function.

Answer:

A. Aperture