Question

multiple choice question
which method begins with placing a small drop of culture on solid medium and involves a series of thinning steps with a sterile inoculation loop to allow the growth of individual colonies?
○ pour plating
○ spread plating
○ streak plating

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Explanation:

• Pour plating: Involves mixing culture with molten agar before pouring into plates, not starting with a drop on solid medium and thinning steps with a loop.
• Spread plating: Uses a spreader to distribute a culture (usually from a liquid) on solid medium, not the series of thinning steps with an inoculation loop as described.
• Streak plating: Begins with placing a small drop of culture on solid medium and then using a sterile inoculation loop to perform a series of thinning (streaking) steps to obtain individual colonies by diluting the culture across the plate.

Answer:

C. Streak plating