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Question
the segment of dna shown in the figure above has restriction sites i and ii, which create restriction fragments a, b, and c. which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?
In gel - electrophoresis of DNA, smaller fragments move faster towards the positive electrode. Fragment C is the largest as it is not cut by either restriction site, fragment A is the smallest as it is the most cut - off piece. So, fragments should be separated with C being closest to the negative electrode and A being closest to the positive electrode.
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The gel with fragments arranged as C, B, A from the negative to the positive electrode.