Question

summarize the steps in rdna (use gene of interest, plasmid dna, restriction enzymes, ligases).

Explanation:

The process involves cutting, mixing, and joining using restriction enzymes and ligase to create rDNA.

Answer:

1. Isolate the gene of interest from the source DNA using restriction enzymes. These enzymes cut the DNA at specific recognition sites.
2. Cut the plasmid DNA with the same restriction enzymes used for the gene - of - interest. This creates compatible sticky or blunt ends.
3. Mix the gene of interest and the cut plasmid DNA. The complementary ends of the gene and the plasmid will base - pair.
4. Add DNA ligase to the mixture. Ligase catalyzes the formation of phosphodiester bonds, joining the gene of interest to the plasmid DNA, creating recombinant DNA (rDNA).