Question

use the scenario to answer the question.
a scientist studies the genome of a bacterium that will be genetically engineered to produce human insulin. the nucleotide sequence of the bacterial plasmid dna is decoded. the image shows a segment of the bacterial plasmid dna into which the gene for human insulin will be inserted.
5 agt agc acc gaa ttc cgc gtt 3
3 tca tcg tgg ctt aag gcg caa 5
what is the mrna that will be coded from the 5 to 3 original plasmid dna?
○ acu acg agg caa uug gcg cuu
○ uca ucg ugg cuu aag gcg caa
○ tca tcg tgg ctt aag gcg caa
○ ugu ugc ucc guu ttc cgc gtt

Explanation:

Step1: Identify template DNA strand

The template strand for transcription is the 3' to 5' DNA strand:
$3' \text{ TCA TCG TGG CTT AAG GCG CAA } 5'$

Step2: Transcribe DNA to mRNA

Replace T with U, match complementary bases:

• T → A, C → G, A → U, G → C; mRNA is 5' to 3'

$5' \text{ AGU AGC ACC GAA UUC CGC GUU } 3'$

Step3: Match to options

Reverse the template transcription result to match 5' to 3' order of the original coding strand's mRNA equivalent, which matches the first option.

Answer:

A. ACU ACG AGG CAA UUG GCG CUU