Question

which of the proteins are directly responsible for ensuring that eukaryotic dna is replicated precisely once in each round of replication, even though there are thousands of replication origins?
dna primase
dna polymerase δ
initiator protein
replication licensing factor
single - strand - binding proteins

Explanation:

• DNA primase synthesizes RNA primers for replication start, not controlling replication frequency.
• DNA polymerase δ is responsible for lagging strand DNA synthesis, not regulating replication once per cycle.
• Initiator proteins trigger the start of replication but do not enforce the once-per-cycle rule.
• Replication licensing factors bind to replication origins only after mitosis, marking them as eligible for replication once per cell cycle, ensuring eukaryotic DNA replicates precisely once per round.
• Single-strand-binding proteins stabilize unwound DNA strands during replication, no role in replication frequency control.

Answer:

replication licensing factor