Question

you have successfully focused on an object with the 10x and 40x objective lenses. you have rotated the 100x objective lens into position but are unable to obtain a sharp image by rotating the fine focus adjustment knob. which of the following is most likely to correct this problem? multiple choice use the coarse focus adjustment knob to sharpen the image. add a drop of immersion oil before rotating the 100x lens into position. decrease the light intensity.

Explanation:

When using a 100x objective lens on a microscope, immersion oil is typically required. It increases the refractive index between the lens and the specimen, allowing more light to enter the lens and improving resolution. Not using immersion oil can lead to an out - of - focus image that cannot be corrected by fine focus adjustments. Using the coarse focus with high - power lenses can damage the lens or specimen. Decreasing light intensity will not fix a focus issue.

Answer:

B. Add a drop of immersion oil before rotating the 100x lens into position.