Question

1. how do you calculate the magnification on a light microscope? magnification = objective × eyepiece magnification. 2. explain how to focus a microscope using the high - power lens. include any safety issues you need to be aware of. focus on low - power first. switch to high - power. use fine adjustment knob. keep equipment clean. avoid lens - slide contact. 3. compare your observations of micrographs obtained from the optical microscope, the scanning electron microscope, and the transmission electron microscope. optical microscopes have lower resolution. sem shows surface details, tem shows internal details. 4. which type of microscope would be the best tool to use to view the surface of a bacterial cell? why?

Explanation:

1. First, start with low - power lens to locate the specimen and get a general view. This helps in finding the area of interest quickly and safely.
2. Then, switch to high - power lens for detailed observation. Use the fine adjustment knob to get a sharp image. Keep the equipment clean to avoid damage and ensure accurate results. Avoid touching the lens with the slide to prevent scratches.
3. Optical microscopes have lower resolution compared to electron microscopes. SEM (Scanning Electron Microscope) is best for viewing the surface of a bacterial cell as it provides detailed surface images. TEM (Transmission Electron Microscope) shows internal details but is not suitable for surface - only viewing.

Answer:

1. To focus a microscope using the high - power lens: First, focus on low - power. Then switch to high - power and use the fine adjustment knob. Keep equipment clean and avoid lens - slide contact.
2. Magnification on a light microscope is calculated as Magnification = Objective x Eyepiece magnifications.
3. When comparing observations from optical, transmission electron, and scanning electron microscopes, optical microscopes have lower resolution. SEM is for surface details and TEM is for internal details.
4. The best tool to view the surface of a bacterial cell is SEM because it provides detailed surface images while optical microscopes have lower resolution and TEM shows internal details.