Question

ligation
restriction enzyme digest
transformation
transduction
question 5
what would happen if you did a re digest with an enzyme that had a cut site in the mid
cloning would fail
cloning and gene expression would work
expression would work by cloning would fail
cloning would work by expression may fail
submit assignment

Explanation:

If a restriction enzyme cuts in the middle of the target gene during digestion, the gene will be fragmented. A broken, non-intact gene cannot be successfully cloned, so the cloning process will fail. Gene expression is irrelevant here as the gene itself is destroyed before cloning can occur.

Answer:

Cloning would fail