QUESTION IMAGE
Question
multiple choice question
in fluorescence microscopy, the ______ absorbs light energy from the excitation light and emits colored light.
cover slip
fluorochrome
antibody
glass slide
Fluorochromes are fluorescent dyes that absorb excitation light energy and emit longer-wavelength (colored) light, which is the core functional component for fluorescence microscopy. Cover slips and glass slides are just mounting supports, and antibodies only carry fluorochromes but do not perform the light absorption-emission function themselves.
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B. fluorochrome