Question

objectives :

1. to determine the abo blood types of four unknown simulated blood samples.
2. to determine the rh of four unknown simulated blood samples.

procedure:

1. pre - label each of your blood typing slides as follows:

slide #1: mr. smith slide #2: ms. jones slide #3: mr. green slide #4: ms. brown

1. place 2 drops of mr. smith’s blood in each of the a, b and rh wells of slide #1.
2. place 2 drops of ms. jones’ blood in each of the a, b and rh wells of slide #2.
3. place 2 drops of mr. green’s blood in each of the a, b and rh wells of slide #3.
4. place 2 drops of ms. brown’s blood in each of the a, b and rh wells of slide #4.
5. add 2 drops of the simulated anti - a serum to each a well on the slide. mix with a toothpick for 10 seconds.
6. add 2 drops of the simulated anti - b serum to each b well on the slide. mix with a toothpick for 10 seconds.
7. add 2 drops of the simulated anti - rh serum to each rh well on the slide. mix with a toothpick for 10 seconds.

if there is any positive reaction in the liquids, (clumping, grainy, etc....) mark a “+” in the box.
if the mixed liquids show no change, mark a “—” to show a negative reaction (no reaction).
data table 1
agglutination reactions

	anti - a serum	anti - b serum	anti - rh serum	blood type	observations (what does it look like?)
sample #2: ms. jones	—	+	—	b -	foam
sample #3: mr. green	+	+	+	ab+	light
sample #4: ms. brown	—	—	—	o -	dark

abo blood groups - review questions: answer using complete sentences

1. what abo antigens are present on the red blood cells of mr. green’s blood?
2. what abo antibodies are present in the plasma of mr. green’s blood?
3. if ms. jones needed a transfusion, what abo type(s) of blood could she safely receive?
4. if ms. brown were serving as a donor, what abo blood type(s) could receive her blood safely?
5. why is it necessary to match the donor’s and the recipient’s blood before a transfusion is given?
6. what happens to red blood cells that are agglutinated?

Explanation:

Question 1:

Mr. Green's blood type is AB+ (from the data table). In the ABO system, blood type AB has both A and B antigens on the red blood cells. So the A and B antigens are present.

For blood type AB, the plasma does not have anti - A or anti - B antibodies. This is because if it had anti - A or anti - B, those antibodies would react with the A and B antigens on its own red blood cells. So Mr. Green (blood type AB+) has neither anti - A nor anti - B antibodies in his plasma.

Ms. Jones has blood type B - (from the data table). People with blood type B can receive blood from type B (since it has the same B antigen, and no A antigen to react with anti - A in their plasma) and type O (since O has no A or B antigens to trigger an immune response). Also, since her Rh factor is negative, she should receive Rh - blood. But for ABO compatibility, the safe ABO types are B and O.

Answer:

The ABO antigens present on the red blood cells of Mr. Green’s blood are A antigen and B antigen.

Question 2: