Question

protein synthesis review
directions: use the dna strand below to:

1. create a complementary strand of dna using base - pairing rules.
2. fill in the correct mrna bases by transcribing the bottom dna code.
3. translate the mrna codons and find the correct amino acid using your codon

chart.

1. answer the questions that follow.

1.
2.
3.

Explanation:

Step1: Complement DNA base pairing

DNA base pairs: A↔T, G↔C. Match each top strand base.
Top DNA (5'→3'): $A-T-G-C-C-A-T-C-G-A-T-T-G-G-A-A-G-A-T-A-A$
Complementary DNA (3'→5'): $T-A-C-G-G-T-A-G-C-T-A-A-C-C-T-T-C-T-A-T-T$

Step2: Transcribe to mRNA

mRNA uses U instead of T; pair with bottom DNA.
Bottom DNA (3'→5'): $T-A-C-G-G-T-A-G-C-T-A-A-C-C-T-T-C-T-A-T-T$
mRNA (5'→3'): $A-U-G-C-C-A-U-C-G-A-U-U-G-G-A-A-G-A-U-A-A$

Step3: Group mRNA into codons

Split mRNA into 3-base codons.
Codons: $AUG, CCA, UCG, AUU, GGA, AGA, UAA$

Step4: Match codons to amino acids

Use standard codon chart:

• $AUG$ = Methionine (Met)
• $CCA$ = Proline (Pro)
• $UCG$ = Serine (Ser)
• $AUU$ = Isoleucine (Ile)
• $GGA$ = Glycine (Gly)
• $AGA$ = Arginine (Arg)
• $UAA$ = Stop (termination)

Answer:

1. Complementary DNA strand (3'→5'): $T-A-C-G-G-T-A-G-C-T-A-A-C-C-T-T-C-T-A-T-T$
2. mRNA strand (5'→3'): $A-U-G-C-C-A-U-C-G-A-U-U-G-G-A-A-G-A-U-A-A$
3. Amino acid sequence: Methionine (Met) → Proline (Pro) → Serine (Ser) → Isoleucine (Ile) → Glycine (Gly) → Arginine (Arg) → Stop