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a cell culture hood is being used to study the lymphocytic choriomeningitis virus (lcmv) outbreak in tamarind monkeys. monkey cells are being cultured in cell culture flasks. syringes are shown attached to filters that would allow the isolation of viruses. a zoom - in window on the flask reveals cells that are at late stages of lcmv infection. in the second zoom - in window on the cells, a new viral particle is shown budding off a cell (right). the researchers needed to determine which cells were appropriate to use in the flask. what cell characteristics did they need to consider and what would they look for to determine if their viral culture attempt was successful?
For cell characteristics, they'd consider cell - type (susceptible to LCMV), growth rate, and stability. To determine success, they'd look for viral replication signs like new viral particles budding off, cytopathic effects (CPE) in cells, and detection of viral genetic material or proteins.
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Cell characteristics: Susceptibility to LCMV, growth rate, stability. Signs of successful viral culture: New viral particles budding off, cytopathic effects, detection of viral genetic material or proteins.