Question

a researcher designs an experiment to investigate the effect of environmental temperature on the function of an enzyme. for each trial included in the experiment, the researcher will add the enzyme and its substrate to an aqueous buffer solution and then measure the amount of product formed over 20 minutes. which of the following must remain the same for all trials of this experiment? a the initial concentration of the substrate b the final concentration of the product c the three - dimensional structure of the enzyme d the temperature of the aqueous buffer solution

Explanation:

In a controlled experiment to study the effect of environmental temperature on enzyme - function, the independent variable is temperature. To accurately determine the effect of temperature, other factors that could influence the reaction need to be kept constant. The initial substrate concentration is a crucial factor that must be the same for all trials to ensure that any differences in product formation are due to temperature changes and not substrate availability. The final product concentration will vary depending on the reaction progress which is affected by temperature. The three - dimensional structure of the enzyme may change with temperature (denaturation). The temperature of the buffer solution is the variable being manipulated.

Answer:

A. The initial concentration of the substrate