Question

the segment of dna shown in the figure above has restriction sites i and ii, which create restriction fragments a, b, and c. which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?

Explanation:

Step1: Recall electrophoresis principle

In gel - electrophoresis of DNA, smaller fragments move faster towards the positive electrode. Fragment C is the largest as it is the non - cut part of the DNA segment, followed by A and then B which is the smallest. So, C will move the least distance, A will move a bit more and B will move the most towards the positive electrode.

Answer:

The gel where the fragments are arranged from left (negative electrode) to right (positive electrode) as C, A, B (the first option in the image where C is closest to the negative end and B is closest to the positive end) is the correct one.