Question

u1l5: microscopes 2 homework:

1. which statement best describes the procedure for removing excess methylene blue (stain) from a wet mount slide preparation?

a. remove the coverslip and drop water onto the specimen.
b. place a piece of paper towel at one edge of the coverslip to absorb the methylene blue, and then add water at the opposite edge of the coverslip
c. insert a dropper under the coverslip and withdraw some methylene blue
d. remove the coverslip, allow the methylene blue to dry, and then replace the coverslip.

1. in a minimum of three steps, describe how to make a proper wet mount of the letter \e\ in your own words.

Explanation:

Question 1

To remove excess methylene blue from a wet mount slide preparation, the standard procedure is to use a paper towel at one edge of the coverslip to draw out the excess stain (by capillary action) and then add water at the opposite edge to replace the removed stain and keep the slide wet. Option a is about adding water to the specimen (not removing excess stain), option c is about adding methylene blue (not removing excess), option d is incorrect as removing the coverslip would disrupt the mount. Option b describes the correct method of using a paper towel at one edge to absorb excess and adding water at the opposite edge.

Step1: Prepare the slide

Place the letter "e" (on a slide or thin paper) on a clean microscope slide.

Step2: Add a drop of water

Put a drop of water (or mounting medium) over the letter "e" to make it a wet mount.

Step3: Apply the coverslip

Hold a coverslip at an angle (using forceps or fingers) and gently lower it over the drop of water and the letter "e", starting from one edge to avoid trapping air bubbles.

Answer:

b. Place a piece of paper towel at one edge of the coverslip to absorb the methylene blue, and then add water at the opposite edge of the coverslip

Question 2