Question

2 using the following nucleotide sequence, predict the complementary strand sequence 5-ttaaacccgttggaac-3 5-aatttgggcaaccttg-3 3-ccgggtttaccaaggt-5 3-ttaaacccgttggaac-5 3-aatttgggcaaccttg-5

Explanation:

Step1: Recall base - pairing rules

In DNA, A (adenine) pairs with T (thymine) and C (cytosine) pairs with G (guanine). The complementary strand runs in the 3' - 5' direction while the given strand is in 5' - 3' direction.

Step2: Pair the nucleotides

For the sequence 5'-TTAAACCCGTTGGAAC-3', the complementary nucleotides are:
T pairs with A, T with A, A with T, A with T, C with G, C with G, C with G, G with C, T with A, T with A, G with C, A with T, A with T, C with G.
The complementary strand in 3' - 5' direction is 3'-AATTTGGGCAACCTTG-5'

Answer:

3'-AATTTGGGCAACCTTG-5'