Question

1. what is the dilution factor if you add 100ul of protease solution to 300 ul of diluent? hint: pay attention in the final volume.

a. 1/1 b. 1/2 c. 1/3 d. 1/4

1. what is the dilution factor if you add 50ul of protease solution to 100 ul of diluent?

a. 1/1 b. 1/2 c. 1/3 d. 1/4

1. what is the dilution factor if you add 50ul of protease solution to 50 ul of diluent?

a. 1/1 b. 1/2 c. 1/3 d. 1/4

Explanation:

Step1: Recall dilution - factor formula

Dilution factor = $\frac{\text{Volume of sample}}{\text{Total volume}}$

Step2: Solve question 5

Volume of sample = 100 μL, Volume of diluent = 300 μL, Total volume = 100 + 300=400 μL. Dilution factor = $\frac{100}{400}=\frac{1}{4}$

Step3: Solve question 6

Volume of sample = 50 μL, Volume of diluent = 100 μL, Total volume = 50 + 100 = 150 μL. Dilution factor = $\frac{50}{150}=\frac{1}{3}$

Step4: Solve question 7

Volume of sample = 50 μL, Volume of diluent = 50 μL, Total volume = 50+50 = 100 μL. Dilution factor = $\frac{50}{100}=\frac{1}{2}$

Answer:

1. d. 1/4
2. c. 1/3
3. b. 1/2