Question

which indicator will not work at room temperature and must be heated first?
○ biurets solution
○ sudan iii solution
○ lugols iodine
○ benedicts solution

Explanation:

Benedict's solution is used to test for reducing sugars. It requires heating to react with reducing sugars and produce a color - change (from blue to green, yellow, orange or red depending on the amount of reducing sugar). Biuret's solution tests for proteins at room temperature, Sudan III solution tests for lipids at room temperature, and Lugol's iodine tests for starch at room temperature.

Answer:

D. Benedict's Solution