Question

why dont we add the enzyme into the master mix?
a) the enzyme takes the most volume and will dilute the reaction
b) the enzyme contains an inhibitor so it will not work
c) the master mix cannot contain proteins as they interfere with measurements
d) the reaction will start immediately and our measurement will be inaccurate

Explanation:

Enzymes are biological catalysts that initiate and speed up biochemical reactions immediately upon contact with their substrates (which are part of the master mix). If added to the master mix upfront, the reaction starts right away, making it impossible to accurately measure the reaction rate or progress at the intended start time, leading to inaccurate data. The other options are incorrect: enzymes typically make up a small volume, they do not inherently contain inhibitors that render them non-functional, and proteins (like enzymes) do not universally interfere with measurements in such assays.

Answer:

d) The reaction will start immediately and our measurement will be inaccurate