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Question
identify effective techniques for accurate pipet use. select all that apply. select one or more: measure liquid by aligning the meniscus with the volume line. do not let liquid enter the pipet bulb or pump. use the pipet bulb to force the last drop out of the tip. leave any air bubbles in a pipet that occur after drawing up liquid.
Brief Explanations
- For "Measure liquid by aligning the meniscus with the volume line": This is a standard technique for accurate pipetting as the meniscus alignment ensures the correct volume is measured.
- For "Do not let liquid enter the pipet bulb or pump": Allowing liquid into the bulb or pump can contaminate the equipment and affect future measurements, so this is a good practice.
- For "Use the pipet bulb to force the last drop out of the tip": Pipets are designed so that the last drop is part of the calibrated volume and should not be forced out, so this is incorrect.
- For "Leave any air bubbles in a pipet that occur after drawing up liquid": Air bubbles affect the volume accuracy, so they should be removed, making this incorrect.
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A. Measure liquid by aligning the meniscus with the volume line, B. Do not let liquid enter the pipet bulb or pump